Detection of Macrolide Resistant Mycoplasma pneumoniae in Children with Lower Respiratory Tract Infection by Sanger Sequencing Targeting Domain V Region of 23S rRNA Gene

Mycoplasma pneumoniae is recognized as the leading cause of community-acquired lower respiratory tract infection in children, accounting for a significant proportion pediatric mortality. Macrolides are first-line treatment M. infections. However, extensive use macrolides clinical practice resulted emergence macrolide-resistant (MRMP), which has negative impact on outcomes. Hence, present study, MRMP was determined hospitalized children with pneumonia (CAP). Real-time PCR detected 41 348 samples. Sanger sequencing revealed that none isolates were associated A2063G or A2064G base mutation, confers macrolide resistance, domain V 23S rRNA gene. Although not observed CAP our healthcare practitioners should be vigilant about potential risk